Question

Activity Prepare a temporary mount of one drop of pond water on a glass slide. Observe it under low power and high power microscope.

Answer 1

here are the instructions for preparing a temporary mount of pond water on a glass slide and observing it under a microscope, written in the second person:

Materials needed:

• Glass slide
• Cover slip
• Pond water
• Dropper
• Microscope
• Lens paper or a clean cloth

Steps:

1. Clean your glass slide and cover slip using lens paper or a clean cloth to remove any dust or debris.

2. Using a dropper, collect one drop of pond water and place it in the center of your glass slide.

3. Carefully place the cover slip on top of the water droplet, making sure there are no air bubbles trapped underneath.

4. Gently press down on the cover slip to spread the water droplet and to remove any excess water around the edges.

5. Place your prepared slide on the microscope stage and secure it in place.

6. Using the low power objective lens, observe your slide under the microscope and focus the image by adjusting the coarse and fine focus knobs.

7. Observe the different structures and organisms present in the pond water, such as algae, protozoa, and microinvertebrates.

8. Switch to the high power objective lens and adjust the focus as necessary to observe finer details and structures in the sample.

Tips:

• Be careful not to crush or damage any organisms when preparing the slide.
• Use a dropper or pipette to collect the water sample to avoid contamination from your fingers or other sources.
• Dispose of your slide and cover slip properly after use to prevent contamination or spread of any microorganisms.